Difference between revisions of "Part:BBa K1499400:Design"
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===Design Notes=== | ===Design Notes=== | ||
The sequence for this gene was obtained from the <i>Polistes dominula</i> genome[1]. We codon-optimized it for expression in <i>E. coli</i> and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies. | The sequence for this gene was obtained from the <i>Polistes dominula</i> genome[1]. We codon-optimized it for expression in <i>E. coli</i> and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies. | ||
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+ | <b>Note:</b> This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality. | ||
===Source=== | ===Source=== |
Revision as of 17:11, 24 October 2014
Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this gene was obtained from the Polistes dominula genome[1]. We codon-optimized it for expression in E. coli and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.
Note: This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality.
Source
Polistes dominula genome.
References
Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB
Studies of amino acid composition of nest protein in other Polistes species: http://link.springer.com/article/10.1007/PL00001733 http://link.springer.com/article/10.1007/s101640070003 http://link.springer.com/article/10.1007/BF00997166 http://link.springer.com/article/10.1007/BF00982111