Difference between revisions of "Part:BBa K1499251"
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<partinfo>BBa_K1499251 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1499251 SequenceAndFeatures</partinfo>
  
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==Characterization==
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===Verification===
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The part was sequence verified in the pSB1C3 backbone before submission to the registry. One forward read was obtained using VF2.
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[[Image:|700px|thumb|center|<b>Figure 2.</b> GFP with two amber stop codons matches the expected sequence.]]
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===Results===
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This part was verified to work by the GFP and aeBlue generator constructs. See this part's experience page for these results.
  
 
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<partinfo>BBa_K1499251 parameters</partinfo>
 
<partinfo>BBa_K1499251 parameters</partinfo>
 
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==Characterization==
 
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===Verification of Part===
 
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==References==
 
==References==
 
1. Thorbjarnardóttir, S ''et al.'' (1985) Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. ''J. Bacteriol.'' 161: 219–22. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/2981802 2981802].
 
1. Thorbjarnardóttir, S ''et al.'' (1985) Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. ''J. Bacteriol.'' 161: 219–22. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/2981802 2981802].

Revision as of 22:27, 2 November 2014

supP tRNA

This part encodes the amber suppressing tRNA supP, a mutated form of the leucine tRNA LeuX.

Usage and Biology

There have been a variety of tRNA mutations found in nature that allow translation of stop codons. These are known collectively as "nonsense suppressors" because the bacteria they are found in are susceptible to infection by viruses with nonsense mutations. This leucine tRNA supP differs from the endogenous leucine tRNA LeuX only in the anticodon and is able to recognize the amber stop codon (UAG) and insert a leucine at that position.[1] This part contains the supP tRNA along with 100 bases upstream and 50 bases downstream to allow for proper tRNA expression and processing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Verification

The part was sequence verified in the pSB1C3 backbone before submission to the registry. One forward read was obtained using VF2.

[[Image:|700px|thumb|center|Figure 2. GFP with two amber stop codons matches the expected sequence.]]

Results

This part was verified to work by the GFP and aeBlue generator constructs. See this part's experience page for these results.

References

1. Thorbjarnardóttir, S et al. (1985) Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. J. Bacteriol. 161: 219–22. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/2981802 2981802].