Difference between revisions of "Part:BBa K1499205:Design"
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===Source=== | ===Source=== | ||
| − | + | ''Deinococcus radiodurans'' genomic DNA, then site-directed mutagenesis for a single base mutation. | |
===References=== | ===References=== | ||
Latest revision as of 22:19, 2 November 2014
MntH with amber stop codon with promoter for expression, no tRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 215
Illegal NgoMIV site found at 1007
Illegal NgoMIV site found at 1080 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The MntH was reisolated from Deinococcus radiodurans genomic DNA. Then, we used site-directed mutagenesis to introduce the stop codon in the gene. A TTG (leucine) codon was mutated to a TAG amber stop codon.
Source
Deinococcus radiodurans genomic DNA, then site-directed mutagenesis for a single base mutation.