Difference between revisions of "Part:BBa K1499004:Design"

(→‎References)
 
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===Source===
 
===Source===
  
−
Streptomyces avidinii (streptavidin domain) and Clostridium cellulovorans (CBD domain)
+
''Streptomyces avidinii'' (streptavidin domain) and ''Clostridium cellulovorans'' (Cellulose binding domain)
 +
Synthesized using Integrated DNA Technlologies and cloned into biobrick backbone.
  
 
===References===
 
===References===
 
1. Tormo, J. <i>et al.</i> (1999) Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. <i>EMBO Journal</i> 15(21) : 5739-5751. PMID: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC452321/pdf/emboj00021-0005].<br>
 
1. Tormo, J. <i>et al.</i> (1999) Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. <i>EMBO Journal</i> 15(21) : 5739-5751. PMID: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC452321/pdf/emboj00021-0005].<br>

Latest revision as of 18:46, 2 November 2014

Cellulose binding domains with streptavidin domain generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 703
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 304
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 651


Design Notes

We used different sequences for cellulose-binding domains to prevent homologous recombination and other issues. We also removed cut sites for RFC-10 compatibility.


Source

Streptomyces avidinii (streptavidin domain) and Clostridium cellulovorans (Cellulose binding domain) Synthesized using Integrated DNA Technlologies and cloned into biobrick backbone.

References

1. Tormo, J. et al. (1999) Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO Journal 15(21) : 5739-5751. PMID: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC452321/pdf/emboj00021-0005].