Difference between revisions of "Part:BBa K1499003:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1499003 short</partinfo>
 
<partinfo>BBa_K1499003 short</partinfo>
  
 
<partinfo>BBa_K1499003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1499003 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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todo
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This gene was isolated from pure ''P. fluorescens'' DNA using the following primers:
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wssI F: 5'-CCGGGTTTGTGTTGTCGTTC-3'
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wssI R: 5'-AGGCAGTCTGCTTCAAGCTC-3'
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The BioBrick prefix and suffix were then added using these primers:
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F: 5'-GAATTCGCGGCCGCTTCTAGAGCCGGGTTTGTGTTGTCGTTC-3'
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R: 5'-CTGCAGCGGCCGCTACTAGTAAGGCAGTCTGCTTCAAGCTC-3'
  
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Site-directed mutagenesis was performed to eliminate the BioBrick restriction site found in this gene using the following primers:
  
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Imut F: 5'-GGACGGCGGCGAGTTCTCCGGTGCGGCCAAGGCG-3'
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Imut R: 5'-CCGCACCGGAGAACTCGCCGCCGTCCTTGCTGAAATTGCCCACAG-3'
  
 
===Source===
 
===Source===
  
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todo
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The wss operon was extracted from ''P. fluorescens'', an organism that produces cellulose acetate as part of a biofilm that allows it to colonize the air-liquid interface [1].
  
 
===References===
 
===References===
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[1] Spiers AJ ''et al.'' (2013) Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads in ''Cellulose - Medical, Pharmaceutical, and Electronic Applications''. DOI: [http://cdn.intechopen.com/pdfs-wm/45637.pdf 10.5772/53736]

Revision as of 04:44, 24 October 2014

wssI gene from wss operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 228
    Illegal NgoMIV site found at 628
    Illegal NgoMIV site found at 961
    Illegal AgeI site found at 275
    Illegal AgeI site found at 919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 850

Design Notes

This gene was isolated from pure P. fluorescens DNA using the following primers:

wssI F: 5'-CCGGGTTTGTGTTGTCGTTC-3' wssI R: 5'-AGGCAGTCTGCTTCAAGCTC-3'

The BioBrick prefix and suffix were then added using these primers:

F: 5'-GAATTCGCGGCCGCTTCTAGAGCCGGGTTTGTGTTGTCGTTC-3' R: 5'-CTGCAGCGGCCGCTACTAGTAAGGCAGTCTGCTTCAAGCTC-3'

Site-directed mutagenesis was performed to eliminate the BioBrick restriction site found in this gene using the following primers:

Imut F: 5'-GGACGGCGGCGAGTTCTCCGGTGCGGCCAAGGCG-3' Imut R: 5'-CCGCACCGGAGAACTCGCCGCCGTCCTTGCTGAAATTGCCCACAG-3'

Source

The wss operon was extracted from P. fluorescens, an organism that produces cellulose acetate as part of a biofilm that allows it to colonize the air-liquid interface [1].

References

[1] Spiers AJ et al. (2013) Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads in Cellulose - Medical, Pharmaceutical, and Electronic Applications. DOI: [http://cdn.intechopen.com/pdfs-wm/45637.pdf 10.5772/53736]