Difference between revisions of "Part:BBa K1499001:Design"
Line 12: Line 12:
 
F: 5'-GAATTCGCGGCCGCTTCTAGAGCCATCGGCCTACGGTAAAGG-3'
 
F: 5'-GAATTCGCGGCCGCTTCTAGAGCCATCGGCCTACGGTAAAGG-3'
 
R: 5'-CTGCAGCGGCCGCTACTAGTATGAGGCAAAGACCATGGTGG-3'
 
R: 5'-CTGCAGCGGCCGCTACTAGTATGAGGCAAAGACCATGGTGG-3'
 +
 +
Site-directed mutagenesis was performed to eliminate the BioBrick restriction site found in this gene using the following primers:
 +
Gmut F:
 +
Gmut R:
  
 
===Source===
 
===Source===

Revision as of 04:45, 24 October 2014

wssG gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This gene was isolated from pure P. fluorescens DNA using the following primers: wssG F: 5'-CCATCGGCCTACGGTAAAGG-3' wssG R: 5'-TGAGGCAAAGACCATGGTGG-3'

The BioBrick prefix and suffix were then added using these primers: F: 5'-GAATTCGCGGCCGCTTCTAGAGCCATCGGCCTACGGTAAAGG-3' R: 5'-CTGCAGCGGCCGCTACTAGTATGAGGCAAAGACCATGGTGG-3'

Site-directed mutagenesis was performed to eliminate the BioBrick restriction site found in this gene using the following primers: Gmut F: Gmut R:

Source

The wss operon was extracted from P. fluorescens, an organism that produces cellulose acetate as part of a biofilm that allows it to colonize the air-liquid interface [1].

References

[1] Spiers AJ et al. (2013) Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads in Cellulose - Medical, Pharmaceutical, and Electronic Applications. DOI: [http://cdn.intechopen.com/pdfs-wm/45637.pdf 10.5772/53736]