Difference between revisions of "Part:BBa K1484314"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1484314 short</partinfo>
 
<partinfo>BBa_K1484314 short</partinfo>
  
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MoClo slot for pro+5U
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Modular Cloning level 1 input cassette, enables insertion of Promoter+ 5'UTR to express GAL4
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This part contains no promoter: prior to use, it must be assembled using BsaI with a level 0 Promoter+5'UTR compatible with the PlantSyntax (RFC105).
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It can be directly transformed (either by ATM or crossing) into plants with circuits driven by the GAL4 Upstream Activation Site (UAS).
  
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Alternatively, it can be assembled using BpiI into modular cloning multigene constructs with circuits driven by the UAS for direct transformation.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 18:02, 10 October 2014

Input module for mosbi

Modular Cloning level 1 input cassette, enables insertion of Promoter+ 5'UTR to express GAL4


Usage and Biology

This part contains no promoter: prior to use, it must be assembled using BsaI with a level 0 Promoter+5'UTR compatible with the PlantSyntax (RFC105).

It can be directly transformed (either by ATM or crossing) into plants with circuits driven by the GAL4 Upstream Activation Site (UAS).

Alternatively, it can be assembled using BpiI into modular cloning multigene constructs with circuits driven by the UAS for direct transformation. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 28
    Illegal BsaI.rc site found at 18
    Illegal SapI site found at 233