Difference between revisions of "Part:BBa K1484106"

Latest revision as of 17:53, 10 October 2014

LTI membrane localisation tag Localisation tag for plant reporters, which localises them to the cell membrane. Compatible with Plant Syntax (RFC105) as a C-Terminal fusion.

Usage and Biology

Add this tag to the 3' of your ORF. IMPORTANT: this contains flanking restriction sites for use with RFC105 compatible assembly.

When used in conjunction with fluorescent reporters, individual cell outlines are clearly visible with 5x magnification.

This tag can be very useful for counting cells, measuring cell size using automated computational techniques. See [Fernan Nature] for an illustration of how this tag can be used.

This tag is effective in many plant chassis:

In Marchantia, the fluorescent reporter appears as follows:

Simple routines can collect information about the sample cell sizes (in pixels).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 45
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 45
Illegal NotI site found at 18
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 45
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 45
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 45
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 233

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1484106 short</partinfo>
+Localisation tag for plant reporters, which localises them to the cell membrane. Compatible with Plant Syntax (RFC105) as a C-Terminal fusion.
+===Usage and Biology===
+Add this tag to the 3' of your ORF. IMPORTANT: this contains flanking restriction sites for use with RFC105 compatible assembly.
-localise to membrane, works with fluorescent proteins. MoClo compatible
+When used in conjunction with fluorescent reporters, individual cell outlines are clearly visible with 5x magnification.
-<!-- Add more about the biology of this part here
+This tag can be very useful for counting cells, measuring cell size using automated computational techniques. See [Fernan Nature] for an illustration of how this tag can be used.
-===Usage and Biology===
+This tag is effective in many plant chassis:
+In Marchantia, the fluorescent reporter appears as follows:
+Simple routines can collect information about the sample cell sizes (in pixels).
 <!-- -->