Difference between revisions of "Part:BBa K1484102"
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This part has been codon optimized for plant expression.
 
This part has been codon optimized for plant expression.
  
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===Usage and Biology===
 
===Usage and Biology===
 
HAP1 is a transcription activator which is active in many eukaryotes. It can be used to amplify sensitive inputs.
 
HAP1 is a transcription activator which is active in many eukaryotes. It can be used to amplify sensitive inputs.

Revision as of 12:11, 10 October 2014

HAP1-VP16 Transcription Activator CDS

HAP1 transcription activator CDS. Binds to its UAS (upstream activation site) for activation of reporter expression in eukaryotes. This part has been codon optimized for plant expression.


Usage and Biology

HAP1 is a transcription activator which is active in many eukaryotes. It can be used to amplify sensitive inputs.

By using an enhancer trap with HAP1, lines can be produced with very specific expression patterns (for example, expression active in a particular tissue). Further circuits utilising reporters driven by the UAS then inherit expression patterns. For example uses, see the use of the alternative transcription activator GAL4 BBa_K1484102

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 236
    Illegal BsaI.rc site found at 535