Difference between revisions of "Part:BBa K1477030:Experience"
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===Characterization and Applications of BBa_K1477030=== | ===Characterization and Applications of BBa_K1477030=== | ||
| − | [[File:Electrophoresis_K1477030.png]] | + | <p> |
| + | [[File:Electrophoresis_K1477030.png|200px|thumb|left|alt text]] | ||
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In correspondence with a 500 basepair DNA ladder, our part measures at ~2500 base pairs which corresponds with the sum of the BioBricks and the pSB1C3 backbone. Plasmid DNA isolated from two subclones | In correspondence with a 500 basepair DNA ladder, our part measures at ~2500 base pairs which corresponds with the sum of the BioBricks and the pSB1C3 backbone. Plasmid DNA isolated from two subclones | ||
</p> | </p> | ||
<p> | <p> | ||
| − | [[File:Coliphage_lysis_of_BBa_K1477030.png]] | + | [[File:Coliphage_lysis_of_BBa_K1477030.png|400px|thumb|left|alt text]] |
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This immediate-lysis device was designed to be an immediate lysis cassette for T7 infection of transformed Top10 cells. The expected result was that a culture of transformed cells would be resistant to infection by T7 phage but not by an unrelated phage. This experiment represents transformed cells infected at a ratio of 100 cells to 1 pfu T7 phage. If functioning, the immediate lysis cassette should have stopped the propagation of T7 infection. What may be lacking is the presence of holin that increase the effectiveness of the endolysin. | This immediate-lysis device was designed to be an immediate lysis cassette for T7 infection of transformed Top10 cells. The expected result was that a culture of transformed cells would be resistant to infection by T7 phage but not by an unrelated phage. This experiment represents transformed cells infected at a ratio of 100 cells to 1 pfu T7 phage. If functioning, the immediate lysis cassette should have stopped the propagation of T7 infection. What may be lacking is the presence of holin that increase the effectiveness of the endolysin. | ||
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===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1477030 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1477030 StartReviews</partinfo> | ||
Revision as of 01:24, 3 November 2014
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Characterization and Applications of BBa_K1477030
In correspondence with a 500 basepair DNA ladder, our part measures at ~2500 base pairs which corresponds with the sum of the BioBricks and the pSB1C3 backbone. Plasmid DNA isolated from two subclones
TEST FOR IMMEDIATE LYSIS OF TOP10 CELLS TRANSFORMED WITH BBa_K1477030. This immediate-lysis device was designed to be an immediate lysis cassette for T7 infection of transformed Top10 cells. The expected result was that a culture of transformed cells would be resistant to infection by T7 phage but not by an unrelated phage. This experiment represents transformed cells infected at a ratio of 100 cells to 1 pfu T7 phage. If functioning, the immediate lysis cassette should have stopped the propagation of T7 infection. What may be lacking is the presence of holin that increase the effectiveness of the endolysin.
User Reviews
UNIQ1f2e71c4477cff3e-partinfo-00000000-QINU UNIQ1f2e71c4477cff3e-partinfo-00000001-QINU