Difference between revisions of "Part:BBa K1467204:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1467204=== | ===Applications of BBa_K1467204=== | ||
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| + | <u> NRP-UEA iGEM 2014 </u> | ||
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| + | The part was used by iGEM14_NRP-UEA-Norwich as a reporter to test several different promoters with the aim of determining which would be the most useful in further stages of the project. Golden Gate cloning was used to hook GFP up to the promoters 35s (BBa_K1467101), BS3 (BBa_K1467102), PDF1.2 (BBa_K1467103) and PR1 to produce transcriptional units that expressed GFP in Nicotiana Benthamiana following infiltration of agrobacterium carrying the relevant plasmid. | ||
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| + | [[Image: GFP expression.jpg|250px|]] | ||
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| + | Nicotiana Benthamiana leaves under UV light following infiltration of agrobacterium carrying the constructs (A) 35s_GFP_ocs, (B) BS3_GFP_ocs, (C) PDF1.2_GFP_ocs. In each case GFP protein has be transcribed and is shown as florescence under UV light following activation of the promoter (35s is constitutive, BS3 has been induced by the TALE AvrBS3 and PDF1.2 has been induced by Methyl Jasmonate). | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:31, 13 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1467204
NRP-UEA iGEM 2014
The part was used by iGEM14_NRP-UEA-Norwich as a reporter to test several different promoters with the aim of determining which would be the most useful in further stages of the project. Golden Gate cloning was used to hook GFP up to the promoters 35s (BBa_K1467101), BS3 (BBa_K1467102), PDF1.2 (BBa_K1467103) and PR1 to produce transcriptional units that expressed GFP in Nicotiana Benthamiana following infiltration of agrobacterium carrying the relevant plasmid.
Nicotiana Benthamiana leaves under UV light following infiltration of agrobacterium carrying the constructs (A) 35s_GFP_ocs, (B) BS3_GFP_ocs, (C) PDF1.2_GFP_ocs. In each case GFP protein has be transcribed and is shown as florescence under UV light following activation of the promoter (35s is constitutive, BS3 has been induced by the TALE AvrBS3 and PDF1.2 has been induced by Methyl Jasmonate).
User Reviews
UNIQ9d7ce8a67a923ac6-partinfo-00000000-QINU UNIQ9d7ce8a67a923ac6-partinfo-00000001-QINU