Difference between revisions of "Part:BBa K1467202"

Revision as of 17:57, 10 October 2014

GoldenGate compatible AmilGFP, Yellow Chromoprotein

Variation of the part BBa_K592010 that has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.

iGEM14_NRP-UEA-Norwich chose to use this part as one of their reporters as the chromoprotein naturally exhibits strong yellow color when expressed. The construct was cloned to be part of a transcriptional unit with MAS as the promoter and octopine synthase (Agrobacterium tumefaciens) as the terminator.

The next stage of The Green Canary Project would be to use this part alongside the part AmilCP (BBa_K1467201) as the two reporters in a multi-gene system with the aim of producing a plant that turns yellow on activation of the promoter..... , induced by the pepper pathogen (TALE) AvrBS3. (Similarly, the blue chomoprotein would be expressed on activation of the promoter by the TALE AvrXa27). This would allow identification of the presence of this pathogen in the area.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1467202 short</partinfo>
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 Variation of the part BBa_K592010 that has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.
-The chromoprotein naturally exhibits strong yellow color when expressed.
-Codon-optimised for expression in dicotyledenous plants.
+iGEM14_NRP-UEA-Norwich chose to use this part as one of their reporters as the chromoprotein naturally exhibits strong yellow color when expressed. The construct was cloned to be part of a transcriptional unit with MAS as the promoter and octopine synthase (Agrobacterium tumefaciens) as the terminator.
+The next stage of The Green Canary Project would be to use this part alongside the part AmilCP (BBa_K1467201) as the two reporters in a multi-gene system with the aim of producing a plant that turns yellow on activation of the promoter..... , induced by the pepper pathogen (TALE) AvrBS3. (Similarly, the blue chomoprotein would be expressed on activation of the promoter by the TALE AvrXa27). This would allow identification of the presence of this pathogen in the area.