Difference between revisions of "Part:BBa K1453505"

Revision as of 19:13, 16 October 2014

New TAL_5

We design seven new sticky ends which get the least score when judging the similarity.

Our parts can be connected in the right order by golden gate method.

New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.

New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE. The TALE can recognize 14bp DNA sequence:

TTCGATATCAAGCT

Seven sticky ends:

AAAA AGGG GTAC GCTC TTTT TCGA CCCC

If you want to know how we design these ends, please go to see our [http://2014.igem.org/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement project-Part3 TAL improve].

Usage and Biology

Because our TAL improvement is based on the 2012 Freiburg's project, you can just follow [http://2012.igem.org/Team:Freiburg/Project/Tal 2012 Freiburg's protocol ]to connect whole TALE.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1453505 short</partinfo>
-s
+We design seven new sticky ends which get the least score when judging the similarity.
+Our parts can be connected in the right order by golden gate method.
+New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.
+New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE. The TALE can recognize 14bp DNA sequence:
+TTCGATATCAAGCT
+Seven sticky ends:
+AAAA	AGGG	GTAC	GCTC	TTTT	TCGA	CCCC
+If you want to know how we design these ends, please go to see our [http://2014.igem.org/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement project-Part3 TAL improve].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Because our TAL improvement is based on the 2012 Freiburg's project, you can just follow [http://2012.igem.org/Team:Freiburg/Project/Tal 2012 Freiburg's protocol ]to connect whole TALE.
 <!-- -->