Difference between revisions of "Part:BBa K1453500"

Line 8: Line 8:
 
New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.
 
New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.
  
New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE.
+
New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE. The TALE can recognize 14bp DNA sequence:
 +
 
 +
TTCGATATCAAGCT
 +
 
 +
 
  
 
Seven sticky ends:
 
Seven sticky ends:

Revision as of 18:58, 16 October 2014

New TAL_left_right

We design seven new sticky ends which get the least score when judging the similarity.

Our parts can be connected in the right order by golden gate method.

New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.

New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE. The TALE can recognize 14bp DNA sequence:

TTCGATATCAAGCT


Seven sticky ends:

AAAA AGGG GTAC GCTC TTTT TCGA CCCC

If you want to know how we design these ends, please go to see our [http://2014.igem.org/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement project-Part3 TAL improve].

Usage and Biology

Because our TAL improvement is based on the 2012 Freiburg's project, you can just follow [http://2012.igem.org/Team:Freiburg/Project/Tal 2012 Freiburg's protocol ]to connect whole TALE.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 27
    Illegal NotI site found at 489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal BamHI site found at 463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]