Difference between revisions of "Part:BBa K1379007:Design"
 
Line 9: Line 9:
  
 
===Source===
 
===Source===
−
 
+
- - P<sub>comFA</sub> primers sequence was obtained from [https://www.sanger.ac.uk/ Wellcome Trust Sanger Institute], a British genomics and genetics research institute.<br>
−
- Phelicase primers sequence was obtained from [https://www.sanger.ac.uk/ Wellcome Trust Sanger Institute], a British genomics and genetics research institute.
+
- iGEM 2014 Hong_Kong_HKUST Team has cloned P<sub>omFA</sub> from S. pneumoniae strain NCTC7465 by PCR. Then, restriction-ligation with &sigma;<sup>X</sup> generator [[Part:BBa_K1379006|BBa_K1379006]] and [[Part:BBa_E0240|BBa_E0240]] in [[Part:pSB1C3|pSB1C3]] backbone was performed.
−
- both sigmaX gene and Phelicase promoter were cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain<br>
+
−
- Constitutive promoter + RBS (BBa_K880005) was obtained from iGEM distribution kit 2014
+
−
- Double terminator (BBa_B0015) was obtained from iGEM distribution kit 2013
+
−
- GFP generator (BBa_E0240) was obtained from iGEM distribution kit 2014
+
  
 
===References===
 
===References===

Latest revision as of 05:01, 12 October 2014

σx Generator + PcomFA-E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359
    Illegal BsaI.rc site found at 1550


Design Notes

None

Source

- - PcomFA primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute.
- iGEM 2014 Hong_Kong_HKUST Team has cloned PomFA from S. pneumoniae strain NCTC7465 by PCR. Then, restriction-ligation with σX generator BBa_K1379006 and BBa_E0240 in pSB1C3 backbone was performed.

References