Difference between revisions of "Part:BBa K1372000"

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This BB is the result of the assembly of two BB:
 
This BB is the result of the assembly of two BB:
 
   
 
   
- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).  
+
- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).
 +
 
- BBa_K228001(lien): for the transcription of SupD-tRNA.  
 
- BBa_K228001(lien): for the transcription of SupD-tRNA.  
  
[[File:Paris Saclay BB2000.png|center]]
+
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:09, 17 October 2014

salicylate inducible suppressing system

This BB is the result of the assembly of two BB:

- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).

- BBa_K228001(lien): for the transcription of SupD-tRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312