Difference between revisions of "Part:BBa K1351036:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1351036 short</partinfo>
 
<partinfo>BBa_K1351036 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
This part contains the native sequences from [i]Staphylococcus aureus[/i] N315.
+
This part contains the native sequences from ''Staphylococcus aureus'' N315.
  
  
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
This part is derived from [i]Staphylococcus aureus[/i] N315 gDNA by PCR using primers with modified RFC25 prefix and suffix:
+
This part is derived from ''Staphylococcus aureus'' N315 gDNA by PCR using primers with modified RFC25 prefix and suffix:
  
 
agrA-fwd: gatcgaattccgcggccgcttctagataaggaggagccggcATGAAAATTTTCATTTGCGAAGACG
 
agrA-fwd: gatcgaattccgcggccgcttctagataaggaggagccggcATGAAAATTTTCATTTGCGAAGACG

Revision as of 17:11, 16 October 2014

Quorum sensing two component system (AIP-II sensing histidine kinase agrC, response regulator agrA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1139
    Illegal AgeI site found at 1114
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475


Design Notes

This part contains the native sequences from Staphylococcus aureus N315.


Source

This part is derived from Staphylococcus aureus N315 gDNA by PCR using primers with modified RFC25 prefix and suffix:

agrA-fwd: gatcgaattccgcggccgcttctagataaggaggagccggcATGAAAATTTTCATTTGCGAAGACG agrA-rev: gatcctgcagcggccgctactagtattaaccggtTATTTTTTTAACGTTTCTCACCG agrC-fwd: gatcgaattccgcggccgcttctagataaggaggagccggcTTGATTCCAACTTTTTCATCTATC agrC-rev: gatcctgcagcggccgctactagtattaaccggtGTTGTTAATAATTTCAACTTTTTGAATAAAG


References