Difference between revisions of "Part:BBa K1351036"

Revision as of 17:08, 16 October 2014

Quorum sensing two component system (AIP-II sensing histidine kinase agrC, response regulator agrA)

Quorum sensing two component system derived from Staphylococcus aureus N315. This part consists of the AIP-II sensing histidine kinase AgrC and the response regulator AgrA. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the P2 and P3 promoters. This part should only work for AIP-II. Other AIPs should lead to inhibition.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1139
Illegal AgeI site found at 1114
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 475

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 __NOTOC__
 <partinfo>BBa_K1351036 short</partinfo>
-Quorum sensing two component system derived from [i]Staphylococcus aureus[/i] N315. This part consists of the AIP-II sensing histidine kinase agrC and the response regulator agrA. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the P2 and P3 promoters. This part should only work for AIP-II. Other AIPs should lead to inhibition.
+Quorum sensing two component system derived from ''Staphylococcus aureus'' N315. This part consists of the AIP-II sensing histidine kinase AgrC and the response regulator AgrA. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the [https://parts.igem.org/Part:BBa_K1351037 P2] and [https://parts.igem.org/Part:BBa_K1351038 P3] promoters. This part should only work for AIP-II. Other AIPs should lead to inhibition.