Difference between revisions of "Part:BBa K1351021"
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Excitation peak: 584 nm Emission peak: 607 nm
 
Excitation peak: 584 nm Emission peak: 607 nm
  
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[[File:LMU14_E1010mut-Pellet|thumb|right|the left eppendorf tube contains a pink bacterial pellet of ''E. coli'' containing the BioBrick BBa_K1351021 in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1351040 pBS0K-pPspac], a ''B. subtilis'' expression vector. On the right, you can see ''E. coli'' with the empty vector]]
  
  

Revision as of 14:26, 29 October 2014

Monomeric Red Fluorescent Protein from Discosoma striata

Mutant of the Biobrick BBa_E1010 [[1]]. In order to make it compatible with the Freiburg-Standard RFC25, two AgeI-Restriction sites were deleted.

monomeric RFP: Red Fluorescent Protein.

Excitation peak: 584 nm Emission peak: 607 nm


File:LMU14 E1010mut-Pellet
the left eppendorf tube contains a pink bacterial pellet of E. coli containing the BioBrick BBa_K1351021 in pBS0K-pPspac, a B. subtilis expression vector. On the right, you can see E. coli with the empty vector


This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:

prefix with EcoRI, NotI, XbaI, SD and NgoMIV: GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]