CWB domain of B. subtilis minor autolysin LytE
Cell wall binding domain and signal peptide (amino acids 1 to 207) of Bacillus subtilis minor autolysin LytE (probable peptidoglycan endopeptidase, Uniprot P54421). This domain has been used for displaying beta-lactamase on the cell surface of B. subtilis (Chen et. al., 2008). Used in the BaKillus project to display pathogen-specific peptides to mediate adhesion of B. subtilis to pathogens.
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:||GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC|
|suffix with AgeI, SpeI, NotI and PstI:||ACCGGTTAATACTAGTAGCGGCCGCTGCAG|
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 459