Difference between revisions of "Part:BBa K1323022"
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SodA is a ribosome binding site that has been shown to be effective at generating fluorescent reporter signal in S.aureus/S.epidermidis hosts (Malone et al., 2007). SarA P1 promoter with SodA RBS generates higher level of protein expression than SarA P1 promoter with SarA RBS ribosome binding site (Malone et al., 2007). Sequence is taken from Malone et al., 2009 paper and synthesized by Biobasic. | SodA is a ribosome binding site that has been shown to be effective at generating fluorescent reporter signal in S.aureus/S.epidermidis hosts (Malone et al., 2007). SarA P1 promoter with SodA RBS generates higher level of protein expression than SarA P1 promoter with SarA RBS ribosome binding site (Malone et al., 2007). Sequence is taken from Malone et al., 2009 paper and synthesized by Biobasic. | ||
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Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent Reporters for Staphylococcus aureus. Journal of Microbiological Methods, 77(3): 251-260. | Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent Reporters for Staphylococcus aureus. Journal of Microbiological Methods, 77(3): 251-260. | ||
Revision as of 13:41, 17 October 2014
Ribosome binding site from the sodA Staphylococcus aureus gene
SodA is a ribosome binding site that has been shown to be effective at generating fluorescent reporter signal in S.aureus/S.epidermidis hosts (Malone et al., 2007). SarA P1 promoter with SodA RBS generates higher level of protein expression than SarA P1 promoter with SarA RBS ribosome binding site (Malone et al., 2007). Sequence is taken from Malone et al., 2009 paper and synthesized by Biobasic.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent Reporters for Staphylococcus aureus. Journal of Microbiological Methods, 77(3): 251-260.
Franke, G. C., Dobinsky, S., Mack, D., Wang, C. J., Sobottka, I., Christner, M., Knobloch, J. K., Horstkotte, M. A., Aepfelbacher, M., & Rohde, H. (2007). Expression and functional characterization of gfpmut3.1 and its unstable variants in Staphylococcus epidermidis. Journal of Microbiological methods 71, 123-132.