Difference between revisions of "Part:BBa K1323018"

Revision as of 04:39, 17 October 2014

oriV from the Staphylococcus aureus pSK41 Plasmid

This construct contains the origin of replication (oriV) from pSK41, a multiresistance plasmid from Staphylococcus aureus. This plasmid is low copy number and undergoes theta-mode of replication (Berg et al., 1998). pSK41 was first found in methicillin-resistant S. aureus (Kwong, 2006). The oriV is part of a replication region that spans a 1.25 kb section (nt 12916-14164) on the pSK41.

References

Berg, T. et al. (1998) Complete nucleotide sequence of pSK1: Evolution of staphylococcal conjugative multiresistance plasmids. J. Bacteriol. 180:4350-4359.

Kwong, S., Skurray, R., Firth, N. (2006). Replication control of staphylococcal multiresistance plasmid pSK41: an antisense RNA mediates dual-level regulation of Rep expression. Journal of Bacteriology, 188(12), 4404-4412.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This construct contains the origin of replication (oriV) from pSK41, a multiresistance plasmid from Staphylococcus aureus. This plasmid is low copy number and undergoes theta-mode of replication (Berg et al., 1998). pSK41 was first found in methicillin-resistant S. aureus (Kwong, 2006). The oriV is part of a replication region that spans a 1.25 kb section (nt 12916-14164) on the pSK41.
+==References==
+Berg, T. et al. (1998) Complete nucleotide sequence of pSK1: Evolution of staphylococcal conjugative multiresistance plasmids. J. Bacteriol. 180:4350-4359.
+Kwong, S., Skurray, R., Firth, N. (2006). Replication control of staphylococcal multiresistance plasmid pSK41: an antisense RNA mediates dual-level regulation of Rep expression. Journal of Bacteriology, 188(12), 4404-4412.