Difference between revisions of "Part:BBa K1321116:Design"

(→‎Source)
m (→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
CBD10+PhytoC  with LacI
+
The fusion protein was cloned together using the NgoMIV and AgeI sites.
−
 
+
  
 +
The promotor was cloned onto the protein via the XbaI/SpeI sites.
  
 
===Source===
 
===Source===

Latest revision as of 20:20, 24 October 2014


Linker-cipA-linker + phytochelatin (PC) EC20 with LacI promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 383
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 383
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated.

References