Difference between revisions of "Part:BBa K1314015"

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===Modelling Results===
 
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Revision as of 18:36, 25 October 2014

Homologous stable integration for nifH promoter T7 expression system Tookit

Homologous stable integration for nifH promoter T7 expression system Tookit


Usage and Biology

EcoRI PstI double digest check gel photo:

Modelling Results



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2742
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2742
    Illegal BamHI site found at 2588
    Illegal XhoI site found at 1387
    Illegal XhoI site found at 2413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2742
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2742
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2757
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 223
    Illegal AgeI site found at 335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.