Difference between revisions of "Part:BBa K1184000:Design"

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===References===
 
===References===
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<p><sup>1</sup> Sergei Pletnev, Nadya G. Gurskaya, Nadya V. Pletneva, Konstantin A. Lukyanov, Dmitri M. Chudakov, Vladimir I. Martynov, Vladimir O. Popov, Mikhail V. Kovalchuk, Alexander Wlodawer, Zbigniew Dauter, and Vladimir Pletnev.<br />
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<p><sup>1</sup> Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A. <br />
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<i>Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed</i><br />
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Journal of Biological Chemistry 2009 284: 32028-32039. First Published on September 8, 2009, doi:10.1074/jbc.M109.054973
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−
</p>
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<p><sup>2</sup> Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A. <br />
+
 
<i>A Genetically Encoded Photosensitizer</i><br />
 
<i>A Genetically Encoded Photosensitizer</i><br />
 
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175
 
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175
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</p>
 +
<p><sup>2</sup> Sergei Pletnev, Nadya G. Gurskaya, Nadya V. Pletneva, Konstantin A. Lukyanov, Dmitri M. Chudakov, Vladimir I. Martynov, Vladimir O. Popov, Mikhail V. Kovalchuk, Alexander Wlodawer, Zbigniew Dauter, and Vladimir Pletnev.<br />
 +
<i>Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed</i><br />
 +
Journal of Biological Chemistry 2009 284: 32028-32039. First Published on September 8, 2009, doi:10.1074/jbc.M109.054973
 
</p>
 
</p>

Revision as of 20:37, 4 September 2013


KillerRed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151
    Illegal BsaI.rc site found at 442


Design Notes

This sequence is not codon optimized for E. coli. Sequence contains 14 rare proline codons (CCC) and a rare arginine codon (AGA).

KillerRed has catalytic residues E70 and S121. The chromophore forms from the backbone of Q67, Y68 and G69.1,2

Source

Engineered from anm2CP. Provided by the Bruchez lab at Carnegie Mellon

References

1 Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175

2 Sergei Pletnev, Nadya G. Gurskaya, Nadya V. Pletneva, Konstantin A. Lukyanov, Dmitri M. Chudakov, Vladimir I. Martynov, Vladimir O. Popov, Mikhail V. Kovalchuk, Alexander Wlodawer, Zbigniew Dauter, and Vladimir Pletnev.
Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed
Journal of Biological Chemistry 2009 284: 32028-32039. First Published on September 8, 2009, doi:10.1074/jbc.M109.054973