Difference between revisions of "Part:BBa K1184000:Design"

 
(Design Notes)
Line 9: Line 9:
 
This sequence is not codon optimized for E. coli.
 
This sequence is not codon optimized for E. coli.
  
 
+
Sequence contains 14 rare proline codons (CCC) and a rare arginine codon (AGA).
  
 
===Source===
 
===Source===

Revision as of 19:32, 4 September 2013


KillerRed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151
    Illegal BsaI.rc site found at 442


Design Notes

This sequence is not codon optimized for E. coli.

Sequence contains 14 rare proline codons (CCC) and a rare arginine codon (AGA).

Source

Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon

References