Difference between revisions of "Part:BBa K1159001:Experience"
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<partinfo>BBa_K1159001 AddReview 5</partinfo>
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<I>iGEM TU Eindhoven 2015</I>
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This part works very well. We used NanoLuc in a BRET sensor, connected to OmpX with a BsoBI-linker. When furimazine is added, NanoLuc will work as a donor, which means that the energy of an electron falling back to its ground state is coupled to a transition of an electron in the RET Acceptor from its ground state to the excited state. We used mNeonGreen as acceptor in this BRET sensor. For more information and further characterization, see the Applications section above.
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Revision as of 18:48, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1159001

User Reviews

UNIQ012c02441d6e658a-partinfo-00000000-QINU

•••••

iGEM TU Eindhoven 2015

This part works very well. We used NanoLuc in a BRET sensor, connected to OmpX with a BsoBI-linker. When furimazine is added, NanoLuc will work as a donor, which means that the energy of an electron falling back to its ground state is coupled to a transition of an electron in the RET Acceptor from its ground state to the excited state. We used mNeonGreen as acceptor in this BRET sensor. For more information and further characterization, see the Applications section above.

UNIQ012c02441d6e658a-partinfo-00000002-QINU