Difference between revisions of "Part:BBa K1152015"
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This engineered version of indC BBa_K11520013 contains the T-domain of the plu2642 protein instead of its native T-domain. It still is capable to produce the blue pigment indigoidine, as OD-measurements showed.
 
This engineered version of indC BBa_K11520013 contains the T-domain of the plu2642 protein instead of its native T-domain. It still is capable to produce the blue pigment indigoidine, as OD-measurements showed.
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In the multiple sequence alignment showed below, this variant of indC is named synT3.
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[[image:IndPD_Fig4.png|800px|Multiple Sequence Alignment of the synthetic T-domains we introduced to indC]]
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K1152015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1152015 SequenceAndFeatures</partinfo>
  
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Figure 2 shows the analytical restiction digest we performed for part validation:
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[[File:Heidelberg2013_BBa_testdigest_indigoidine.png|280px|thumb|center|'''Figure 2:''' Restriction digest with EcoRI, XbaI, SpeI, PstI.]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 19:00, 6 October 2013

IndC Indigoidine Synthetase device with T-domain of plu2642

This engineered version of indC BBa_K11520013 contains the T-domain of the plu2642 protein instead of its native T-domain. It still is capable to produce the blue pigment indigoidine, as OD-measurements showed.

In the multiple sequence alignment showed below, this variant of indC is named synT3.

Multiple Sequence Alignment of the synthetic T-domains we introduced to indC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730

Figure 2 shows the analytical restiction digest we performed for part validation:

Figure 2: Restriction digest with EcoRI, XbaI, SpeI, PstI.