Difference between revisions of "Part:BBa K1152012:Experience"

 
m
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
<partinfo>BBa_K1152012 short</partinfo>
−
how you used this part and how it worked out.
+
* CDS of sfp derived from ''Delftia acidovorans'' SPH-1
 +
 
 +
<partinfo>BBa_K1152012 SequenceAndFeatures</partinfo>
  
−
===Applications of BBa_K1152012===
 
  
−
===User Reviews===
+
===Design Notes===
−
<!-- DON'T DELETE --><partinfo>BBa_K1152012 StartReviews</partinfo>
+
The delC CDS is derived from ''Delftia acidovorans'' SPH-1. [Johnston, 2013]
−
<!-- Template for a user review
+
We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (<partinfo>BBa_R0010</partinfo>) and the RBS <partinfo>BBa_B0029</partinfo>.
−
{|width='80%' style='border:1px solid gray'
+
−
|-
+
−
|width='10%'|
+
−
<partinfo>BBa_K1152012 AddReview number</partinfo>
+
−
<I>Username</I>
+
−
|width='60%' valign='top'|
+
−
Enter the review inofrmation here.
+
−
|};
+
−
<!-- End of the user review template -->
+
−
<!-- DON'T DELETE --><partinfo>BBa_K1152012 EndReviews</partinfo>
+

Revision as of 23:22, 5 October 2013

DelC 4'-Phosphopanthetheinyl-transferase

  • CDS of sfp derived from Delftia acidovorans SPH-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 288


Design Notes

The delC CDS is derived from Delftia acidovorans SPH-1. [Johnston, 2013] We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.