Difference between revisions of "Part:BBa K1152012:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1152012 short</partinfo>
 
<partinfo>BBa_K1152012 short</partinfo>
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* CDS of sfp derived from ''Delftia acidovorans'' SPH-1
  
 
<partinfo>BBa_K1152012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1152012 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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We cloned sfp in a pSB3K3-derived plasmid with a lac promoter and the RBS BBa_B0029.
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The delC CDS is derived from ''Delftia acidovorans'' SPH-1. [Johnston, 2013]
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We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (<partinfo>BBa_R0010</partinfo>) and the RBS <partinfo>BBa_B0029</partinfo>.
  
  
  
 
===Source===
 
===Source===
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We amplified delC from Delftia acidovorans SPH-1 obtained from DSMZ.
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We amplified delC from Delftia acidovorans SPH-1
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===References===
 
===References===
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* Johnston, C. W., Wyatt, M. a, Li, X., Ibrahim, A., Shuster, J., Southam, G., & Magarvey, N. a. (2013). Gold biomineralization by a metallophore from a gold-associated microbe. Nature chemical biology, 9(4), 241–243. doi:10.1038/nchembio.1179

Latest revision as of 18:28, 6 October 2013

DelC 4'-Phosphopanthetheinyl-transferase

  • CDS of sfp derived from Delftia acidovorans SPH-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 288


Design Notes

The delC CDS is derived from Delftia acidovorans SPH-1. [Johnston, 2013] We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.


Source

We amplified delC from Delftia acidovorans SPH-1 obtained from DSMZ.

References

  • Johnston, C. W., Wyatt, M. a, Li, X., Ibrahim, A., Shuster, J., Southam, G., & Magarvey, N. a. (2013). Gold biomineralization by a metallophore from a gold-associated microbe. Nature chemical biology, 9(4), 241–243. doi:10.1038/nchembio.1179