Difference between revisions of "Part:BBa K1152012"
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<partinfo>BBa_K1152012 short</partinfo>
 
<partinfo>BBa_K1152012 short</partinfo>
  
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DelC encodes a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.
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[[File:Heidelberg2013_PPtase_entD.png|220px|thumb|left|'''Figure 1:''' Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.]]
  
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[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 1:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
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DelC (Figure 1) encodes a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (Figure 2).
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[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 2:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
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Figure 3 shows the analytical restiction digest we performed for part validation:
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[[File:Heidelberg2013_BBa_testdigest_CDS.png|280px|thumb|center|'''Figure 3:''' Restriction digest with EcoRI, XbaI, SpeI, PstI.]]
  
  

Revision as of 18:28, 6 October 2013

DelC 4'-Phosphopanthetheinyl-transferase

Figure 1: Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.

DelC (Figure 1) encodes a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (Figure 2).

Figure 2: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.


Figure 3 shows the analytical restiction digest we performed for part validation:

Figure 3: Restriction digest with EcoRI, XbaI, SpeI, PstI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 288

References

  • Johnston, C. W., Wyatt, M. a, Li, X., Ibrahim, A., Shuster, J., Southam, G., & Magarvey, N. a. (2013). Gold biomineralization by a metallophore from a gold-associated microbe. Nature chemical biology, 9(4), 241–243. doi:10.1038/nchembio.1179