Difference between revisions of "Part:BBa K1152012"

Revision as of 23:22, 5 October 2013

DelC 4'-Phosphopanthetheinyl-transferase

DelC encodes a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.

Figure 1: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 288

References

Johnston, C. W., Wyatt, M. a, Li, X., Ibrahim, A., Shuster, J., Southam, G., & Magarvey, N. a. (2013). Gold biomineralization by a metallophore from a gold-associated microbe. Nature chemical biology, 9(4), 241–243. doi:10.1038/nchembio.1179

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1152012 short</partinfo>
 DelC encodes a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.
+[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 1:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+DelC is a PPTase of ''Delftia acidovorans'' found by in-silico prediction. [Johnston, 2013]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1152012 SequenceAndFeatures</partinfo>
+===References===
+* Johnston, C. W., Wyatt, M. a, Li, X., Ibrahim, A., Shuster, J., Southam, G., & Magarvey, N. a. (2013). Gold biomineralization by a metallophore from a gold-associated microbe. Nature chemical biology, 9(4), 241–243. doi:10.1038/nchembio.1179
 <!-- Uncomment this to enable Functional Parameter display