Difference between revisions of "Part:BBa K1152011:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1152011 short</partinfo>
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<partinfo>BBa_K1152010 short</partinfo>
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* CDS of entD derived from ''Escherichia coli'' and be used in order to activate an NRPS (e.g. entB) (Atp, 1997)
  
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<partinfo>BBa_K1152011 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1152010 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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We cloned sfp in a pSB3K3-derived plasmid with a lac promoter and the RBS BBa_B0029.
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The entD CDS is derived from ''E. coli'' MG1655.
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We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (<partinfo>BBa_R0010</partinfo>) and the RBS <partinfo>BBa_B0029</partinfo>.
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===Source===
 
===Source===
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We amplified entD from genome of ''E. coli'' MG1655.
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We amplified entD from E. coli MG1655
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===References===
 
===References===
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* Atp, U., Gehring, A. M., Bradley, K. A., & Walsh, C. T. (1997). Enterobactin Biosynthesis in Escherichia coli : Isochorismate Lyase ( EntB ) Is a Bifunctional Enzyme That Is Phosphopantetheinylated by EntD and Then Acylated, 2960(97), 8495–8503.

Revision as of 23:21, 5 October 2013

Svp 4'-Phosphopanthetheinyl-transferase

  • CDS of entD derived from Escherichia coli and be used in order to activate an NRPS (e.g. entB) (Atp, 1997)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 173
    Illegal NgoMIV site found at 413
    Illegal NgoMIV site found at 663
    Illegal AgeI site found at 573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 366


Design Notes

The entD CDS is derived from E. coli MG1655. We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.


Source

We amplified entD from genome of E. coli MG1655.

References

  • Atp, U., Gehring, A. M., Bradley, K. A., & Walsh, C. T. (1997). Enterobactin Biosynthesis in Escherichia coli : Isochorismate Lyase ( EntB ) Is a Bifunctional Enzyme That Is Phosphopantetheinylated by EntD and Then Acylated, 2960(97), 8495–8503.