Difference between revisions of "Part:BBa K1152011"

Latest revision as of 18:24, 6 October 2013

EntD 4'-Phosphopanthetheinyl-transferase

Figure 1: Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.

EntD (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (Figure 2). It is the endogenous PPtases of Escherichia coli, located within the enterobactin pathway.

Figure 2: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Figure 3 shows the analytical restiction digest we performed for part validation:

Figure 3: Restriction digest with EcoRI, XbaI, SpeI, PstI.

Sequence and Features