Difference between revisions of "Part:BBa K1152011"

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<partinfo>BBa_K1152010 short</partinfo>
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<partinfo>BBa_K1152011 short</partinfo>
  
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EntD encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes
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[[File:Heidelberg2013_PPtase_entD.png|220px|thumb|left|'''Figure 1:''' Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.]]
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EntD (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (Figure 2).
 
It is the endogenous PPtases of ''Escherichia coli'', located within the enterobactin pathway.
 
It is the endogenous PPtases of ''Escherichia coli'', located within the enterobactin pathway.
  
 
[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 1:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
 
[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 1:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
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Figure 3 shows the analytical restiction digest we performed for part validation:
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[[File:Heidelberg2013_BBa_testdigest_CDS.png|280px|thumb|center|'''Figure 2:''' Restriction digest with EcoRI, XbaI, SpeI, PstI.]]
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1152010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1152011 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1152010 parameters</partinfo>
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<partinfo>BBa_K1152011 parameters</partinfo>
 
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Revision as of 18:20, 6 October 2013

EntD 4'-Phosphopanthetheinyl-transferase


Figure 1: Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.

EntD (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (Figure 2). It is the endogenous PPtases of Escherichia coli, located within the enterobactin pathway.

Figure 1: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Figure 3 shows the analytical restiction digest we performed for part validation:

Figure 2: Restriction digest with EcoRI, XbaI, SpeI, PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]