Difference between revisions of "Part:BBa K1152011"

 
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<partinfo>BBa_K1152011 short</partinfo>
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<partinfo>BBa_K1152010 short</partinfo>
  
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sfp encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.
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EntD encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes
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It is the endogenous PPtases of ''Escherichia coli'', located within the enterobactin pathway.
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[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 1:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
  
 
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===Usage and Biology===
 
===Usage and Biology===
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* EntD is a PPTase and can be used for activating NRPSs. (own work, [2013.igem.org/Team:Heidelberg/Indigoidine|iGEM Heidelberg2013 - Indigoidine])
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* this part can be used in order to overexpress the endogenous PPTase of ''E. coli''
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1152011 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1152010 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1152011 parameters</partinfo>
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<partinfo>BBa_K1152010 parameters</partinfo>
 
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Revision as of 23:20, 5 October 2013

Svp 4'-Phosphopanthetheinyl-transferase

EntD encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes It is the endogenous PPtases of Escherichia coli, located within the enterobactin pathway.

Figure 1: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 173
    Illegal NgoMIV site found at 413
    Illegal NgoMIV site found at 663
    Illegal AgeI site found at 573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 366