Difference between revisions of "Part:BBa K1151001:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<partinfo>BBa_K1151001 AddReview 1</partinfo>
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<I>Imperial College 2014</I>
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We attempted to use this protein as part of fusions with cellulose binding domains. However after cloning our fusions using this part we noticed that unfortunately this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). To correct the mutation a 'C' needs to be added between bases 52-53. This should be able to be easily achieved using site-directed mutagenesis PCR.
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Revision as of 05:10, 2 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1151001

User Reviews

UNIQ9a64f535a453c5b4-partinfo-00000000-QINU UNIQ9a64f535a453c5b4-partinfo-00000001-QINU


•

Imperial College 2014

We attempted to use this protein as part of fusions with cellulose binding domains. However after cloning our fusions using this part we noticed that unfortunately this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). To correct the mutation a 'C' needs to be added between bases 52-53. This should be able to be easily achieved using site-directed mutagenesis PCR.

;