Difference between revisions of "Part:BBa K1150043"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150043 short</partinfo>
 
<partinfo>BBa_K1150043 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|pH1:tracrRNA pU6:DR_dummy_DR
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|-
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|'''Function'''
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|two small RNAs giving rise to
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specific binding of dCas9 to two VEGF loci
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Organism'''
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|<i>Streptococcus pyogenes</i>
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|-
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|'''Source'''
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|Feng Zhang, Addgene
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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 +
By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the dCas9 derivates at once.
  
 
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Revision as of 14:39, 3 October 2013

uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3

pH1:tracrRNA pU6:DR_dummy_DR
Function two small RNAs giving rise to

specific binding of dCas9 to two VEGF loci

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes
Source Feng Zhang, Addgene
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]



Usage and Biology

By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the dCas9 derivates at once.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
    Illegal BglII site found at 1061
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 1053