Difference between revisions of "Part:BBa K1150024:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker | + | For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker separates the dCas9 and the G9a set-domain to minimize the possibility of interference between the domains. |
[[File:Freiburg2013_Plasmid_Cas9-G9a.png|800px|]] <br> | [[File:Freiburg2013_Plasmid_Cas9-G9a.png|800px|]] <br> | ||
'''Figure 1.:''' Complete overview on CMV:dCas9-G9a with all features. | '''Figure 1.:''' Complete overview on CMV:dCas9-G9a with all features. | ||
Latest revision as of 10:24, 3 October 2013
uniCAS Histone Modifier (CMV promoter)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 900
Illegal BglII site found at 5375 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker separates the dCas9 and the G9a set-domain to minimize the possibility of interference between the domains.
Figure 1.: Complete overview on CMV:dCas9-G9a with all features.
Source
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