Difference between revisions of "Part:BBa K1150003"
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<partinfo>BBa_K1150003 short</partinfo> | <partinfo>BBa_K1150003 short</partinfo> | ||
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | ||
! colspan="2" style="background:#FFBF00;"|G9a | ! colspan="2" style="background:#FFBF00;"|G9a | ||
|- | |- | ||
|'''Function''' | |'''Function''' | ||
| − | |Histone | + | |Histone Methyltransferase |
|- | |- | ||
|'''Use in''' | |'''Use in''' | ||
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| − | G9a is ... | + | This part belongs to the uniCAS toolkit of iGEM Team Freiburg 2013 and contains the SET domain of the murine G9a protein. This catalytically active domain is able to transfer methyl groups to lysine 9 of histone 3 (H3K9), which is part of every nucleosome. In regions of open chromatin structures H3K9 methylation can lead to chromatin condensation and thereby inactivation of the concerned locus. This G9a part alone contains the histone methyltransferase domain needed for transcriptional repression and can be combined with dCas9 [[Part:BBa_K1150000]] to target different loci for transcriptional repression of gene expression. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 19:39, 26 September 2013
G9a
| G9a | |
|---|---|
| Function | Histone Methyltransferase |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Organism | Mus musculus |
| Source | Albert Jeltsch, Stuttgart |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
This part belongs to the uniCAS toolkit of iGEM Team Freiburg 2013 and contains the SET domain of the murine G9a protein. This catalytically active domain is able to transfer methyl groups to lysine 9 of histone 3 (H3K9), which is part of every nucleosome. In regions of open chromatin structures H3K9 methylation can lead to chromatin condensation and thereby inactivation of the concerned locus. This G9a part alone contains the histone methyltransferase domain needed for transcriptional repression and can be combined with dCas9 Part:BBa_K1150000 to target different loci for transcriptional repression of gene expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]