Difference between revisions of "Part:BBa K112001:Design"

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===Source===
 
===Source===
 
<pre>
 
<pre>
−
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
+
Construction of {<xis!} Biobrick Part K112201
−
Sub into pBca1256 (EcoRI/BamHI)
+
PCR mea003/mea002 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
−
Product is pBca1256-K112001
+
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112201
 
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----------------------------------------
−
bx3 Forward biobricking of {<T4 Holin!}
+
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
−
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
+
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
−
bx2 Reverse biobricking of {<T4 Holin!}
+
−
cgttaGGATCCttatttagcccttcctaatattctggccgcc
+
 
</pre>
 
</pre>
  
 
===References===
 
===References===

Latest revision as of 01:14, 22 October 2008

Xis from bacteriophage lambda, assembly standard 21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

References