Difference between revisions of "Part:BBa K1106004:Experience"
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| + | <I>Igem BsAs 2013</I> | ||
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'''Objective''' | '''Objective''' | ||
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'''Results''' | '''Results''' | ||
As shown in the graphics below GFP production increases with the arsenite concentration. | As shown in the graphics below GFP production increases with the arsenite concentration. | ||
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| + | '''Conclusion''' | ||
| + | The promoter is sensitive to different arsenite concentrations. | ||
https://static.igem.org/mediawiki/2013/9/96/Gfpinduccion.jpg | https://static.igem.org/mediawiki/2013/9/96/Gfpinduccion.jpg | ||
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https://static.igem.org/mediawiki/2013/e/e3/Gfpampliacion2.jpg | https://static.igem.org/mediawiki/2013/e/e3/Gfpampliacion2.jpg | ||
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Revision as of 22:56, 27 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1106004
User Reviews
UNIQd1ed9978dc73ea64-partinfo-00000000-QINU UNIQd1ed9978dc73ea64-partinfo-00000001-QINU
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Igem BsAs 2013 |
Objective Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations. Methods Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). Results As shown in the graphics below GFP production increases with the arsenite concentration. Conclusion The promoter is sensitive to different arsenite concentrations.
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