Difference between revisions of "Part:BBa K1106000"
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This construction permit to tune the expression of mRFP with the proper construction of LuxR and P22 c2. | This construction permit to tune the expression of mRFP with the proper construction of LuxR and P22 c2. | ||
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+ | {| style="width:100%; background: #CEE3F6" | ||
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+ | |This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a ''cross-feeding'' regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. | ||
+ | |[[Image:Igem.bsas.png | 100px]] | ||
+ | |} | ||
Revision as of 22:02, 27 September 2013
mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed
Monomeric RFP: Red Fluorescent Protein under hybrid promoter activated by HSL-LuxR and repressed by P22 C2.
Usage and Biology
This construction permit to tune the expression of mRFP with the proper construction of LuxR and P22 c2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 650
Illegal AgeI site found at 762 - 1000COMPATIBLE WITH RFC[1000]