Difference between revisions of "Part:BBa K1060009:Experience"

Revision as of 21:11, 4 October 2013

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Characterization of BBa_K1060009

E. coli BL21(DE3) strain

Mg2+: 5mM

control: BL21(DE3) without plasmid and antibiotics

System testing

[http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]

In this video you will find our first behavioural experiment with aphids. An EBF-producing bacterium plate (E. coli BL21(DE3) on LB with 5mM Mg2+ and Chloramphenicol is placed on the left, a control plate on the right, aphids are placed on the leaf in the middle. The first results indicate a positive trend, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.) For more information on the experimental setup and a discussion of the results go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here].

User Reviews

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 [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]
-In this video you will find our first behavioural experiment with aphids. An EBF-producing bacterium plate (''E. coli'' BL21(DE3) on LB with 5mM Mg2+ and Chloramphenicol is placed on the left, a control plate on the right, aphids are placed on the leaf in the middle. The first results indicate a positive trend, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.) For more information on the experimental setup and a discussion of the results go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF here] and scroll down to the aphid experiments section.
+In this video you will find our first behavioural experiment with aphids. An EBF-producing bacterium plate (''E. coli'' BL21(DE3) on LB with 5mM Mg2+ and Chloramphenicol is placed on the left, a control plate on the right, aphids are placed on the leaf in the middle. The first results indicate a positive trend, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.) For more information on the experimental setup and a discussion of the results go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here].
 ===User Reviews===
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