Difference between revisions of "Part:BBa K1060009"
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==System testing== | ==System testing== | ||
| − | + | Our pilot experiment with aphid indicated that our brick performed the desired function, for the video of aphids experiment, please go to [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]. | |
| − | [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video] | + | |
For more infromation see the experience page or go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here]. | For more infromation see the experience page or go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here]. | ||
Revision as of 23:03, 4 October 2013
Medium constitutive expression of EBF synthase from Artemisia annua
For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 177 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1368
Illegal BamHI site found at 677
Illegal XhoI site found at 639 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 249
Construct digestion
System testing
Our pilot experiment with aphid indicated that our brick performed the desired function, for the video of aphids experiment, please go to [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video].
For more infromation see the experience page or go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here].