Difference between revisions of "Part:BBa K1039032"
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The attP site is the phage attachment site for the Bxb1 phage. The site is recognized by Bxb1 integrase. The Bxb1 integrase catalyzes a unidirectional strand exchange between a 53 bp attP site and a 50 bp attB site (Bonnet et al., 2012). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).
 
The attP site is the phage attachment site for the Bxb1 phage. The site is recognized by Bxb1 integrase. The Bxb1 integrase catalyzes a unidirectional strand exchange between a 53 bp attP site and a 50 bp attB site (Bonnet et al., 2012). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).
  
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Reference: Biological Sciences - Applied Biological Sciences - Physical Sciences - Engineering: Jerome Bonnet, Pakpoom Subsoontorn, and Drew Endy. Rewritable digital data storage in live cells via engineered control of recombination directionality. PNAS 2012 ; published ahead of print May 21, 2012, doi:10.1073/pnas.1202344109
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Reference: Jerome Bonnet, Pakpoom Subsoontorn, and Drew Endy. Rewritable digital data storage in live cells via engineered control of recombination directionality. PNAS 2012 ; published ahead of print May 21, 2012, doi:10.1073/pnas.1202344109
  
 
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Revision as of 09:29, 17 September 2013

BXB1 att B

The attP site is the phage attachment site for the Bxb1 phage. The site is recognized by Bxb1 integrase. The Bxb1 integrase catalyzes a unidirectional strand exchange between a 53 bp attP site and a 50 bp attB site (Bonnet et al., 2012). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).

Reference: Jerome Bonnet, Pakpoom Subsoontorn, and Drew Endy. Rewritable digital data storage in live cells via engineered control of recombination directionality. PNAS 2012 ; published ahead of print May 21, 2012, doi:10.1073/pnas.1202344109

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 42
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 22