Difference between revisions of "Part:BBa K1021003"

Revision as of 19:29, 19 October 2016

bar (bialaphos resistance)

The bialaphos resistance (bar) gene confers resistance to bialaphos and phosphinothricin.

Usage and Biology

The common fungus transformation system of entomopathogen Metarhizium anisopliae was based on resistance to the herbicide glufosinate ammonium (phosphinothricin), conferred by the bar gene.

Team NYMU 2016 characterized the bar marker gene, a twin of this part, using the bar expression cassette on vector pBARGPE1 with our chassis organism M.anisopliae.

The transformants carrying the bar gene can grow on the PDA containing glufosinate ammonium.

Figure1. The emerged colonies on glufosinate ammonium agar

Figure2. Transformants grow on selective medium

We did the glufosinate ammonium inhibition test using three different concentrations (0, 160, 1000 ug/mL) with bar transformants and wildtype as a control, the result is showed below.

The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting M.anisopliae conidia cultivation on solid media. The mycelium length was measured on the same line on each plate.

• The higher glufosinate ammonium concentration, the more sparse the mycelium grows outward.

Data:

The mycelium length on 0, 4, 5, 6 days

These results showed that glufosinate ammonium affect the growth of the mycelium, but not as effectively as expected. It may be caused by the degradation of this herbicide due to the long store.

Sequence and Features