Difference between revisions of "Part:BBa K1021003"
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We did the glufosinate ammonium inhibition test using three different concentrations (0, 160, 1000 ug/mL) with transformants with gene ''bar'' and wildtype as a control, the result is showed below.
  
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The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting ''M.anisopliae'' conidia  cultivation on solid media.
  
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[[Image:Bar a.png|left|350px]]
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[[Image:Bar-6.png|center|350px]]
  
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Data:
  
  
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[[Image:Bar data.png|center|350px|thumb|The mycelium length on 0, 4, 5, 6 days]]
  
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[[Image:Bar 1.png|center|350px]]
  
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[[Image:
  
  
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We did the glufosinate ammonium inhibition test, the data is showed below.
 
−
 
−
The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting ''M.anisopliae'' conidia  cultivation on solid media.
 
−
 
−
[[Image:Bar a.png|left|350px]]
 
−
[[Image:Bar-6.png|center|350px]]
 
  
  

Revision as of 19:14, 19 October 2016


bar (bialaphos resistance)

The bialaphos resistance (bar) gene confers resistance to bialaphos and phosphinothricin.

Usage and Biology

The common fungus transformation system of entomopathogen Metarhizium anisopliae was based on resistance to the herbicide glufosinate ammonium (phosphinothricin), conferred by the bar gene.

Team NYMU 2016 characterized the bar marker gene, a twin of this part, using the bar expression cassette on vector pBARGPE1 with our chassis organism M.anisopliae.

The transformants carrying the bar gene can grow on the PDA containing glufosinate ammonium.

Figure1. The emerged colonies on glufosinate ammonium agar
Figure2. Transformants grow on selective medium





We did the glufosinate ammonium inhibition test using three different concentrations (0, 160, 1000 ug/mL) with transformants with gene bar and wildtype as a control, the result is showed below.

The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting M.anisopliae conidia cultivation on solid media.

Bar a.png
Bar-6.png

Data:


The mycelium length on 0, 4, 5, 6 days
Bar 1.png

[[Image:










Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 545
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]