Difference between revisions of "Part:BBa J70004"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70004 short</partinfo>
 
<partinfo>BBa_J70004 short</partinfo>
  
Cloning plasmid used by Geneart for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
+
Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
  
 
Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta).  Vector is AmpR.
 
Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta).  Vector is AmpR.

Revision as of 17:51, 23 January 2008

BioBasic Cloning Plasmid pUC57

Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.

Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal PstI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1790
    Illegal SapI site found at 707