Difference between revisions of "Part:BBa J428387"
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Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI. | Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI. | ||
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| + | Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft. | ||
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Latest revision as of 20:12, 29 June 2022
pJUMP19-Pac Level 0 Promoter acceptor vector with eGFP downstream
pJUMP19-Pac Level 0 Promoter acceptor vector with eGFP downstream
Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.
Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3026
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3032 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3026 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3026
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3026
Plasmid lacks a suffix.
Illegal XbaI site found at 3041
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 853
Illegal NgoMIV site found at 982
Illegal NgoMIV site found at 1476 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2274